anti total erk1 2  (Cell Signaling Technology Inc)

Journal: Pharmaceuticals

Article Title: Olmesartan-Loaded PLGA Nanoparticles Attenuate Methotrexate-Induced Kidney Injury: Association with the AT1R/ERK1/2 Signaling Axis

doi: 10.3390/ph19091462

Figure Lengend Snippet: Effect of OLM and OLM-PLGA on oxidative stress markers ( A ) MDA, ( B ) GSH, ( C ) SOD, and ( D ) NADPH; on inflammatory markers ( E ) TNF-α and ( F ) NF-ĸB; and on the gene expression of ( G ) AT1R against MTX-induced nephrotoxicity in rats. Representative cropped Western blot images showing the expression of p-ERK1/2 with β-actin as a loading control. Results are expressed as Mean ± SEM ( n = 6). Statistical significance was conducted through one-way ANOVA, followed by Tukey’s post hoc test, and shown as: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns = non-significant. MTX: methotrexate, OLM: olmesartan, PLGA: biodegradable poly (lactide-co-glycolic) acid nanoformulation, MDA: malondialdehyde, GSH: reduced glutathione, SOD: superoxide dismutase, NADPH oxidase: nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, TNF-α: tumor necrosis factor-alpha, NF-ĸB p65: phosphorylated nuclear factor-kappa B, AT1R: angiotensin II receptor I.

Article Snippet: The membranes were incubated with the primary antibodies against total ERK1/2 (Cat. No. A4782, ABclonal Biotech Co., Wuhan, China) and p-ERK1/2 (Cat. No. 4370, Cell Signaling Technology, Danvers, MA, USA), followed by incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies.

Techniques: Gene Expression, Western Blot, Expressing, Control

Journal: Pharmaceuticals

Article Title: Olmesartan-Loaded PLGA Nanoparticles Attenuate Methotrexate-Induced Kidney Injury: Association with the AT1R/ERK1/2 Signaling Axis

doi: 10.3390/ph19091462

Figure Lengend Snippet: Effect of OLM and OLM-PLGA on the protein expression of ( A ) total ERK1/2, ( B ) p-ERK1/2, and ( C ) p-ERK1/2/total ERK1/2 ratio against MTX-induced nephrotoxicity in rats. ( D ) Representative cropped Western blot images showing the expression of total and p-ERK1/2, with β-actin as a loading control. Results are expressed as Mean ± SEM ( n = 6). Statistical significance was conducted through one-way ANOVA, followed by Tukey’s post hoc test, and shown as: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns = non-significant. MTX: methotrexate, OLM: olmesartan, PLGA: biodegradable poly (lactide-co-glycolic) acid nanoformulation. ERK1/2: extracellular signal-regulated kinase1/2, p-ERK1/2: phosphorylated extracellular signal-regulated kinase1/2.

Article Snippet: The membranes were incubated with the primary antibodies against total ERK1/2 (Cat. No. A4782, ABclonal Biotech Co., Wuhan, China) and p-ERK1/2 (Cat. No. 4370, Cell Signaling Technology, Danvers, MA, USA), followed by incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies.

Techniques: Expressing, Western Blot, Control

Journal: Frontiers in Veterinary Science

Article Title: Anti-inflammatory, analgesic, and anti-immune-mediated polyarthritis activities of topically applied polarity fractions of Clematis florida var. Plena and their underlying mechanisms

doi: 10.3389/fvets.2026.1846520

Figure Lengend Snippet: Effect of C. florida fractions on the phosphorylation of ERK1/2 and NF-κB p65 in AA rats ( n = 3 per group). (A) Representative Western blot images showing phospho-ERK1/2, total ERK1/2, phospho-NF-κB p65, and total NF-κB p65 in synovial tissues from different experimental groups. (B) Quantification of the phospho-ERK1/2 to total ERK1/2 ratio. (C) Quantification of the phospho-NF-κB p65 to total NF-κB p65 ratio. Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. ## p < 0.01, ### p < 0.001 vs. control group; * p < 0.05, ** p < 0.01 vs. model group.

Article Snippet: The membranes were then incubated overnight at 4 °C with the following primary antibodies: p-ERK1/2 (Thr202/Tyr204; Cell Signaling Technology, USA, #3033S, 1:4000), total ERK1/2 (Cell Signaling Technology, USA, #4695S, 1:1000), NF-κB p65 (phospho-Ser536) (BIOSS, China, bsm-33117M, 1:1000), and NF-κB p65 (total) (ABclonal, China, A4782, 1:2000).

Techniques: Phospho-proteomics, Western Blot, Control

Journal: Frontiers in Veterinary Science

Article Title: Anti-inflammatory, analgesic, and anti-immune-mediated polyarthritis activities of topically applied polarity fractions of Clematis florida var. Plena and their underlying mechanisms

doi: 10.3389/fvets.2026.1846520

Figure Lengend Snippet: Immunohistochemical staining of NF-κB p65 proteins in AA rats. (A) Immunohistochemical staining of p-NF-κB p65 expression. (B) Immunohistochemical staining of total NF-κB p65 expression. (C) Area of p-NF-κB p65 expression on osseous tissue. (D) Area of p-NF-κB p65 expression on synovial tissue. (E) Area of total NF-κB p65 expression on osseous tissue. (F) Area of total NF-κB p65 expression on synovial tissue ( n = 5). All figures were magnified by 200×. Scale bar: 50 μm. Statistical comparisons were performed using one-way ANOVA followed by Dunnett’s post hoc test. # p < 0.05, ### p < 0.001 vs. Mcontrol group; * p < 0.05, ** * p < 0.001 vs. model group.

Article Snippet: The membranes were then incubated overnight at 4 °C with the following primary antibodies: p-ERK1/2 (Thr202/Tyr204; Cell Signaling Technology, USA, #3033S, 1:4000), total ERK1/2 (Cell Signaling Technology, USA, #4695S, 1:1000), NF-κB p65 (phospho-Ser536) (BIOSS, China, bsm-33117M, 1:1000), and NF-κB p65 (total) (ABclonal, China, A4782, 1:2000).

Techniques: Immunohistochemical staining, Staining, Expressing